ʻO Streptomycin ka lāʻau antibiotic mua i ʻike ʻia ma ka papa aminoglycoside a loaʻa mai kahi actinobacterium o kaStreptomycesgenus1. Hoʻohana nui ʻia ia i ka mālama ʻana i nā maʻi bacterial koʻikoʻi i hoʻokumu ʻia e nā maʻi Gram-negative a me Gram-positive, me ka maʻi maʻi maʻi, endocardial a me nā maʻi meningeal a me ke ahulau. ʻOiai ua ʻike ʻia ʻo ka hana mua o ka streptomycin ma o ke kāohi ʻana i ka synthesis protein ma o ka hoʻopaʻa ʻana i ka ribosome, ʻaʻole maopopo ke ʻano o ke komo ʻana i ka cell bacteria.

ʻO ke kahawai mechanosensitive o ka conductance nui (MscL) kahi ala mechanosensitive bacteria i mālama pono ʻia e ʻike pololei i ka ʻāʻī o ka membrane.2. ʻO ka hana physiological o MscL ʻo ia ka pahu hoʻokuʻu pilikia e kū ana i kahi hāʻule nui o ka osmolarity o ke kaiapuni (hypo-osmotic downshock)3. Ma lalo o ke koʻikoʻi hypo-osmotic, komo ka wai i loko o ka cell bacteria e pehu ai, a laila e hoʻonui ai i ka ʻeha i loko o ka membrane; ʻO nā ʻīpuka MscL i ka pane ʻana i kēia haʻalulu e hana ana i kahi pore nui ma kahi o 30 Å4, no laila e ʻae ai i ka hoʻokuʻu wikiwiki ʻana o nā solutes a me ka mālama ʻana i ke kelepona mai ka lysis. Ma muli o ka nui o ka pore, ua hoʻoponopono paʻa ʻia ka MscL gating; ka hōʻike ʻana o kahi alahele MscL hewa ʻole, e wehe ana ma lalo o nā haʻalulu maʻamau, e hoʻolohi i ka ulu ʻana o ka maʻi bacteria a i ʻole ka make cell.5.

Ua manaʻo ʻia nā kaila mechanosensitive bacterial e like me nā pahuhopu lāʻau kūpono no ko lākou kuleana koʻikoʻi i ka physiology o ka bacteria a me ka nele o nā homologues i ʻike ʻia i nā meaola kiʻekiʻe.6. No laila ua hana mākou i kahi pale kiʻekiʻe-throughput (HTS) e ʻimi ana i nā pūhui e pale ai i ka ulu ʻana o ka maʻi bacteria ma kahi ʻano hilinaʻi MscL. ʻO ka mea mahalo, ma waena o nā hits i loaʻa iā mākou ʻehā mau lāʻau lapaʻau i ʻike ʻia, ma waena o lākou ka hoʻohana nui ʻia o nā antibiotics aminoglycosides streptomycin a me spectinomycin.

Aia ka mana o streptomycin i ka ʻōlelo MscL i ka ulu a me nā hoʻokolohua viabilityin vivo.Hāʻawi pū mākou i nā hōʻike o ka hoʻololi pololei ʻana o ka hana MscL channel e dihydrostreptomycin i nā hoʻokolohua patch clamp.in vitro. ʻO ke komo ʻana o MscL i ke ala o ka hana streptomycin e hōʻike ana ʻaʻole wale i kahi mīkini hou no ka loaʻa ʻana o kēia mole nui a polar nui i ke kelepona i nā haʻahaʻa haʻahaʻa, akā nā mea hana hou e hoʻololi i ka mana o nā lāʻau antibiotic i ʻike mua ʻia.