Streptomycin yog thawj cov tshuaj tua kab mob uas tau pom nyob rau hauv cov chav kawm aminoglycoside thiab yog muab los ntawm cov kab mob actinobacterium.Streptomycesgenus1. Nws yog dav siv nyob rau hauv kev kho mob ntawm cov kab mob loj uas tshwm sim los ntawm cov kab mob Gram-negative thiab Gram-positive, nrog rau tuberculosis, endocardial thiab meningeal kab mob thiab kab mob plague. Txawm hais tias nws paub tias lub hauv paus txheej txheem ntawm kev ua ntawm streptomycin yog los ntawm inhibition ntawm protein synthesis los ntawm kev khi cov ribosome, cov txheej txheem ntawm nkag mus rau cov kab mob ntawm tes tseem tsis tau meej.

Mechanosensitive channel ntawm loj conductance (MscL) yog ib qho kev txuag cov kab mob mechanosensitive channel uas ncaj qha hnov ​​​​qhov nro hauv daim nyias nyias.2. Lub luag haujlwm ntawm lub cev ntawm MscL yog qhov kev tso tawm thaum muaj xwm txheej ceev uas nkag mus rau qhov kev poob qis hauv osmolarity ntawm ib puag ncig (hypo-osmotic downshock)3. Nyob rau hauv hypo-osmotic kev ntxhov siab, dej nkag mus rau hauv cov kab mob ntawm tes ua rau nws o, yog li ua kom nruj hauv daim nyias nyias; MscL rooj vag nyob rau hauv teb rau qhov nro no ua ib tug loj pore ntawm txog 30 Å4, yog li tso cai rau kev tso tawm sai sai ntawm cov kua nplaum thiab txuag lub cell ntawm lysis. Vim yog qhov loj pore loj, MscL gating yog nruj tswj; kev nthuav qhia ntawm qhov tsis zoo-gating MscL channel, uas qhib qis dua li ib txwm nruj, ua rau qeeb cov kab mob loj hlob lossis txawm tias cell tuag.5.

Cov kab mob kab mob mechanosensitive tau raug npaj los ua cov hom phiaj tshuaj zoo tshaj vim lawv lub luag haujlwm tseem ceeb hauv kev noj qab haus huv ntawm cov kab mob thiab tsis muaj cov cim homologues hauv cov kab mob siab dua.6. Yog li ntawd peb tau ua qhov kev tshuaj ntsuam siab tshaj plaws (HTS) tshawb nrhiav cov tebchaw uas yuav cuam tshuam cov kab mob loj hlob hauv MscL-raws li. Interestingly, ntawm hits peb pom plaub paub tshuaj tua kab mob, ntawm lawv cov dav siv aminoglycosides tshuaj tua kab mob streptomycin thiab spectinomycin.

Lub zog ntawm streptomycin yog nyob ntawm MscL qhia hauv kev loj hlob thiab kev muaj peev xwm thwmsimhauv vivo.Peb kuj muab pov thawj ntawm kev hloov pauv ncaj qha ntawm MscL channel kev ua haujlwm los ntawm dihydrostreptomycin hauv thaj chaw sim clamphauv vitro. Kev koom tes ntawm MscL nyob rau hauv txoj kev ntawm streptomycin kev txiav txim qhia tsis yog tsuas yog ib tug tshiab mechanism rau yuav ua li cas no bulky thiab heev polar molecule tau nkag mus rau lub cell ntawm tsawg concentrations, tab sis kuj tshiab cov cuab yeej rau modulate potency ntawm twb paub thiab muaj peev xwm tshuaj tua kab mob.