Streptomycin bụ ọgwụ nje mbụ achọtara na klaasị aminoglycoside wee nweta ya site na actinobacterium nkeStreptomycesgenus1. A na-eji ya eme ihe n'ọtụtụ ebe n'ịgwọ ọrịa nje dị njọ nke nje bacteria gram-negative na gram-positive kpatara, gụnyere ụkwara nta, ọrịa endocardial na meningeal na ọrịa. Ọ bụ ezie na a maara na usoro izizi nke streptomycin bụ site na igbochi njikọ protein site na ijikọ ribosome, usoro ntinye na cell nje amabeghị.

Mechanosensitive ọwa nke nnukwu conductance (MscL) bụ ọwa nje mechanosensitive nke echekwara nke ukwuu nke na-achọpụta esemokwu na akpụkpọ ahụ ozugbo.2. Ọrụ physiological nke MscL bụ nke valvụ ntọhapụ mberede nke na-adakwasị nnukwu ọdịda na osmolarity nke gburugburu ebe obibi (hypo-osmotic downshock)3. N'okpuru nrụgide hypo-osmotic, mmiri na-abanye n'ime mkpụrụ ndụ nje na-eme ka ọ zaa, si otú ahụ na-abawanye esemokwu na akpụkpọ ahụ; MscL ọnụ ụzọ ámá nzaghachi a esemokwu akpụ a nnukwu pore nke banyere 30 Å4, si otú a na-enye ohere maka mwepụta ngwa ngwa nke solutes na ịzọpụta cell site na lysis. N'ihi nnukwu pore nha, a na-ahazi MscL gating nke ọma; ngosipụta nke ọwa MscL na-adịghị mma, nke na-emepe na obere esemokwu, na-ebute uto nje ngwa ngwa ma ọ bụ ọbụna ọnwụ cell.5.

Atụpụtala ọwa usoro nje bacteria dị ka ebumnuche ọgwụ dị mma n'ihi ọrụ dị mkpa ha na physiology nke nje bacteria na enweghị ụdị homologues achọpụtara na ihe ndị dị elu.6. Ya mere, anyị rụrụ ihuenyo dị elu (HTS) na-achọ ogige ndị ga-egbochi uto nje n'ụzọ dabere na MscL. N'ụzọ na-akpali mmasị, n'ime hits anyị hụrụ ọgwụ nje anọ a ma ama, n'etiti ha bụ ọgwụ nje aminoglycosides streptomycin na spectinomycin a na-ejikarị eme ihe.

Ike nke streptomycin dabere na nkwupụta MscL na nleba anya uto na ikena vivo.Anyị na-enyekwa ihe akaebe nke mgbanwe kpọmkwem nke ọwa MscL site na dihydrostreptomycin na nnwale mkpọchi.na vitro. Ntinye aka nke MscL n'okporo ụzọ nke streptomycin na-egosi na ọ bụghị naanị usoro ọhụụ maka etu molecule a buru ibu ma dị oke egwu si enweta cell na obere mkpokọta, kamakwa ngwaọrụ ọhụrụ iji gbanwee ike nke ọgwụ nje ama ama na enwere ike.