Streptomycin ndiyo yekutanga mishonga inorwisa mabhakitiriya kuwanikwa mukirasi yeaminoglycoside uye inotorwa kubva kune actinobacterium yeStreptomycesgenus1. Inoshandiswa zvakanyanya mukurapa kwehutachiona hwehutachiona hwakakomba hunokonzerwa neese Gram-negative uye Gram-positive mabhakitiriya, kusanganisira tuberculosis, endocardial uye meningeal zvirwere uye denda. Kunyange zvazvo zvichizivikanwa kuti nzira yekutanga yekuita ye streptomycin ndeyekudzivisa kweprotein synthesis nekusunga ribosome, nzira yekupinda musero rebhakitiriya haisati yanyatsojeka.

Mechanosensitive chiteshi chemaitiro makuru (MscL) inzira yakachengetedzwa yebhakitiriya mechanosensitive chiteshi inonzwa zvakananga kushushikana mu membrane.2. Basa rehupenyu hweMscL nderechimbichimbi yekuburitsa vharafu iyo inovhara pane yakanyanya kudonha muosmolarity yezvakatipoteredza (hypo-osmotic downshock)3. Pasi pe hypo-osmotic stress, mvura inopinda musero yebhakitiriya ichiita kuti iwedzere, nokudaro kuwedzera kukakavara mu membrane; MscL magedhi mukupindura kunetsana uku kuumba pore hombe inosvika makumi matatu Å4, nokudaro kubvumira kukurumidza kusunungurwa kwema solutes uye kuchengetedza sero kubva ku lysis. Nekuda kwehukuru hwepore, MscL gating inodzorwa zvakasimba; kutaura kwemis-gating MscL chiteshi, iyo inovhura yakaderera pane yakajairwa kusawirirana, inokonzera kunonoka kwebhakitiriya kukura kana kunyange sero kufa.5.

Bacterial mechanosensitive channels yakatsanangurwa seyakanaka zvinodhaka zvinonangwa nekuda kwekuita kwavo kwakakosha mufiziology yebhakitiriya uye kushomeka kwemahomologues anozivikanwa muzvisikwa zvepamusoro.6. Saka takaita high-throughput screen (HTS) tichitsvaga mishonga inodzivisa kukura kwebhakitiriya nenzira inotsamira MscL. Sezvineiwo, pakati pezvibodzwa takawana maantibioti mana anozivikanwa, pakati pavo anoshandiswa zvakanyanya aminoglycosides antibiotics streptomycin uye spectinomycin.

Simba re streptomycin rinotsamira pakutaura kweMscL mukukura uye kuyedza kugonain vivo.Isu tinopawo humbowo hweiyo yakananga modulation yeMscL chiteshi chiitiko ne dihydrostreptomycin mune chigamba kuyedza kuedza.in vitro. Kubatanidzwa kweMscL munzira ye streptomycin kunoratidza kwete nzira itsva yekuti iyi yakawanda uye ine polar molecule inowana sei kupinda musero panguva yakaderera, asiwo maturusi matsva ekugadzirisa potency yeanotozivikanwa uye anogona mishonga inorwisa mabhakitiriya.