Streptomycin ke setlhare sa pele sa lithibela-mafu se fumanoeng sehlopheng sa aminoglycoside 'me se nkiloe ho actinobacteria ea likokoana-hloko.Streptomycesleloko1. E sebelisoa haholo ho phekola mafu a tebileng a baktheria a bakoang ke libaktheria tse peli tsa Gram-negative le Gram-positive, ho akarelletsa le lefuba, mafu a endocardial le meningeal le lefu la seoa. Leha ho tsejoa hore mokhoa oa mantlha oa ts'ebetso ea streptomycin ke ka ho thibela protheine synthesis ka ho tlama ribosome, mokhoa oa ho kena seleng ea baktheria ha o so hlake.

Mechanosensitive channel of great conductance (MscL) ke mocha o bolokehileng haholo oa baktheria o utloang tsitsipano ka har'a lera.2. Karolo ea fisioloji ea MscL ke ea valve ea tokollo ea tšohanyetso e koalang ho theoha ho hoholo ha osmolarity ea tikoloho (hypo-osmotic downshock)3. Tlas'a khatello ea kelello ea hypo-osmotic, metsi a kena ka seleng ea baktheria e etsa hore e ruruhe, kahoo e eketsa tsitsipano ka har'a lera; Liheke tsa MscL ka lebaka la tsitsipano ena e etsang pore e kholo e ka bang 30 Å4, kahoo e lumella ho lokolloa ka potlako ha solutes le ho boloka sele ho tloha lysis. Ka lebaka la boholo bo boholo ba pore, MscL gating e laoloa ka thata; Pontšo ea mocha oa MscL o sa sebetseng hantle, o bulang tsitsipano e tlase ho feta e tloaelehileng, o baka kholo ea baktheria e liehang kapa esita le lefu la sele.5.

Mecha ea baktheria ea mechanosensitive e hlahisitsoe e le lipheo tse nepahetseng tsa lithethefatsi ka lebaka la karolo ea tsona ea bohlokoa ho physiology ea libaktheria le khaello ea li-homologues tse tsebahalang linthong tse phahameng.6. Ka hona re ile ra etsa skrine e phahameng ea "high-throughput" (HTS) ho batla metsoako e tla thibela kholo ea baktheria ka mokhoa o itšetlehileng ka MscL. Hoa thahasellisa hore har'a tse otlang re fumane lithibela-mafu tse 'nè tse tsejoang, har'a tsona ke li-antibiotics tse sebelisoang haholo tsa aminoglycoside streptomycin le spectinomycin.

Matla a streptomycin a ipapisitse le polelo ea MscL litekong tsa kholo le ts'ebetsoka vivo.Re boetse re fana ka bopaki ba phetoho e tobileng ea ts'ebetso ea mocha oa MscL ka dihydrostreptomycin litekong tsa patch clamp.ka vitro. Ho kenya letsoho ha MscL tseleng ea ts'ebetso ea streptomycin ha e fane feela ka mokhoa o mocha oa hore na molek'hule ena e ngata haholo le ea polar e fumana monyetla oa ho kena sele ka maemo a tlase, empa hape le lisebelisoa tse ncha tsa ho fetola matla a lithibela-mafu tse seng li tsejoa le tse ka bang teng.