Rabbit coccidiosis ke lefu le atileng hohle le bakoang ke mofuta o le mong kapa ho feta ho tse 16 tsa mofuta oa apicomplexan.Eimeria stiedae.^1-4Matšoao a tloaelehileng a kliniki ea lefu lena a khetholloa ke ho ba lerootho, ho fokotsa tšebeliso ea lijo, letšollo kapa ho patoa, ho atolosoa ha sebete, ascites, icterus, ho senya mpa le lefu.³Coccidiosis ho meutlanyana e ka thibeloa le ho phekoloa ka ho sebelisa lithethefatsi.^1,3,5,6Toltrazuril (Tol), 1-[3-methyl-4-(4-trifluoromethylsulfanyl-phenoxy)-phenyl]-3-methyl-1,3,5-triazin-2,4,6-trione (Setšoantšo sa 1), ke motsoako oa symmetrical triazinetrione o sebelisoang haholo ho thibela le ho loantša coccidiosis.^7-10Leha ho le joalo, ka lebaka la ho fokola ha metsi a qhibilihang, Tol e thata ho monngoa ke pampitšana ea gastrointestinal (GI). Litlamorao tsa bongaka tsa Tol li theotsoe ka lebaka la ho qhibiliha ha eona pampitšaneng ea GI.

Setšoantšo sa 1 Sebopeho sa lik'hemik'hale sa toltrazuril.

Ho qhibiliha ho fokolang ha metsi ha Tol ho hlōtsoe ke mekhoa e meng, e kang ho hasana ho tiileng, matla a ultrafine, le nanoemulsion.^11-13Joalo ka mekhoa e sebetsang ka ho fetesisa ea ho eketsa solubility, Tol solid dispersion e ekelitse feela ho qhibiliha ha Tol ho makhetlo a 2,000,¹¹e bontšang hore ho qhibiliha ha eona ho ntse ho hloka ho ntlafatsoa haholo ka mekhoa e meng. Ho phaella moo, ho qhalakanngoa ho tiileng le nanoemulsion ha ho tsitsa ebile ha ho bonolo ho boloka, ha matla a ultrafine a hloka lisebelisoa tse rarahaneng ho hlahisa.

β-cyclodextrin (β-CD) e sebelisoa haholo ka lebaka la boholo ba eona bo ikhethang ba sekoti, katleho ea ho rarahana ha lithethefatsi, le ho ntlafatsa botsitso ba lithethefatsi, ho qhibiliha, le bioavailability.^14,15Bakeng sa boemo ba eona ba taolo, β-CD e thathamisitsoe mehloling e mengata ea pharmacopoeia, ho kenyelletsa le US Pharmacopoeia/National Formulary, European Pharmacopoeia, le Japanese Pharmaceutical Codex.^16,17Hydroxypropyl–β-CD (HP-β-CD) ke motsoako oa hydroxyalkyl β-CD o ithutoang haholo ka ho kenyelletsa lithethefatsi ka lebaka la bokhoni ba eona ba ho kenyelletsa le ho qhibiliha ha metsi ho phahameng.^18-21Lithuto tsa Toxicologic li tlalehile ka polokeho ea HP-β-CD tsamaisong ea methapo le ea molomo ho 'mele oa motho,²²le HP-β-CD e 'nile ea sebelisoa mefuteng ea meriana ho hlōla mathata a fokolang a ho qhibiliha le ho matlafatsa bioavailability.²³

Ha se litlhare tsohle tse nang le thepa e lokelang ho etsoa e rarahaneng ka HP-β-CD. Tol o ile a fumanoa a e-na le thepa e ipapisitseng le palo e kholo ea mosebetsi oa ho etsa lipatlisiso. Ho eketsa solubility le bioavailability ea Tol ka ho kenyelletsa sebopeho se rarahaneng le HP-β-CD, toltrazuril-hydroxypropyl-β-cyclodextrin inclusion complex (Tol-HP-β-CD) e ile ea lokisetsoa ka mokhoa oa ho hlohlelletsa tharollo thutong ena, 'me e tšesaane. -layer chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy, le nuclear magnetic resonance (NMR) spectroscopy e ile ea sebelisoa ho khetholla Tol-HP-β-CD e fumanoeng. Lintlha tsa pharmacokinetic tsa Tol le Tol-HP-β-CD ka mebutlanyana ka mor'a tsamaiso ea molomo li ile tsa bapisoa le ho feta ka vivo.